1st CLAFCME Cryo - Electron Microscopy Workshop


ORGANIZERS


Dr. Raúl. Padrón

Department of Structural Biology, IVIC
Director of CLAFCME

M.Sc. Fredi Sánchez
Department of Engineering, IVIC


Ing. Fernando. Mendoza

FEI Company
International Coordinator of CLAFCME


Program

September 17 - 20, 2007

Supported by:
CIASEM, CLAFCME - Supramolecular Structure Laboratory - Structural Biology Department - IVIC - CLAB (UNESCO) and FEI Company

with the kind help of Lic.Miriam Ramos (International Coordinator of IVIC, CLAB, tel +58 212 504 1240, fax +58 212 504 1034, e-mail: CLAB@ivic.ve),
Lic. Carmen Delia Flores (Administrador, Department of Structural Biology - IVIC - MCT) and Electronic Engineer Francisco Gerbes (BEINCA-FEI)

Altos de Pipe (IVIC) current weather   Forecast
Departamento de Biología Estructural - IVIC - 1736 m.s.n.m. http://www.yv5fih.org.ve/pipe/gizmo.gif http://www.yv5fih.org.ve/pipe/gizmosolar.gif
http://www.yv5fih.org.ve/pipe/gizmouv.gif
Rango de temperatura actual
Local time = UTC (GMT) - 4 hours (no DST)


Click photo two times to enlarge.

Foto Mardonio Diaz, Servicio de Fotografía Científica del IVIC


Invited
Speakers

Speaker
E-mail

Dr. Wim Busing Wim.Busing@fei.com
Director NanoPort, FEI Company, Eidhoven, The Netherlands
Dr. Wim Voorhout Wim.Voorhout@fei.com Manager AppLab TEM, Sr Applications Specialist NanoBiology,
Applications Laboratory, FEI Company, Eidhoven,
The Netherlands
Ing. Fernando Mendoza Fernando.Mendoza@fei.com Area Manager, Indirect Channels Group, FEI Europe B.V.,
Eidhoven, The Netherlands
International Coordinator of CLAFCME

Dr. Francisco Capani

franciscocapani@hotmail.com

franciscocapani@yahoo.com.ar
Facultad de Medicina, Universidad de Buenos Aires, Argentina
Dr. Domingo Ferrer
dferrer@che.utexas.edu University of Texas at Austin, Texas, U.S.A.
M. Sc. Maristela Granados mgranado@ivic.ve
Department of Structural Biology, IVIC
Lic. Lorenzo Alamo alamo@ivic.ve
Department of Structural Biology, IVIC
Dr. Gustavo Marquez gmarquez@ivic.ve
Department of Structural Biology, IVIC
Ing. Antonio Pinto apinto@ivic.ve
Department of Structural Biology, IVIC
M. Sc. Fredi Sánchez fsanchez@ivic.ve
Department of Structural Biology, IVIC
Dr. Jose Reinaldo Guerrero
guerrero@ivic.ve Department of Structural Biology, IVIC
Dr. Raúl Padrón padron@ivic.ve
Head, Department of Structural Biology, IVIC;
Director of CLAFCME



Participants
Participant
E-mail
Country
Silvia Belmonte
sbelmont@fcm.uncu.edu.ar
Argentina
Amado Quintar
aquintar@cmefcm.uncor.edu
Argentina
Claudio Fader
cfader@fcm.uncu.edu.ar Argentina
Francisco Capani

franciscocapani@hotmail.com

franciscocapani@yahoo.com.ar

Argentina
Kildare Miranda
kmiranda@biof.ufrj.br Brazil
Jonatas Amaral
amaraljb@gmail.com
Brazil
Ingrid Flores

iflores@ivic.ve

ingflorez@hotmail.com
Colombia
Viviana Falcón
viviana.falcon@cigb.edu.cu
vivianit2005@yahoo.com.mx
Cuba
Sandra Rodriguez
sandra.rodriguez@cnic.edu.cu Cuba
Lucero Ruiz
lucpo@colpos.mx Mexico
Armando Zepeda
azr@servidor.unam.mx Mexico
Violeta Garcia
violetaggr@yahoo.es Peru
Alvaro Oliveira
alvarobq@fcien.edu.uy
Uruguay
Domingo Ferrer dferrer@che.utexas.edu U.S.A.
Jose Zambrano
jzambran@ivic.ve Venezuela
Luz Acevedo
luzmacevedo@hotmail.com Venezuela
Reicy Brito
rbrito@ivic.ve 
Venezuela
Ulf Lundberg
ulundber@ivic.ve
Venezuela
Suecia

Program

Monday 17 Tuesday 18 Wednesday 19 Thursday 20
8:00 - 8:45 AM  Breakfast
8:00 - 8:45 AM  Breakfast 8:00 - 8:45 AM  Breakfast 8:00 - 8:45 AM  Breakfast
9:00 -9:45 AM
Wim Busing

Cryo-methods
9:00 - 9:45 AM
Wim Voorhhout
Cryo-preparation methods
Inmunogold
9:00 - 9:45 AM
Wim Voorhout
Tomography
9:00 - 9:45 AM
Raul Padron
Solving biological problems with 3DEM

9:45 - 10:15 AM Coffee break

9:45 - 10:15 AM Coffee break

9:45 - 10:15 AM Coffee break

9:45 - 10:15 AM Coffee break

10:15 - 11:00 AM
Wim Busing
Cryo-TEM



11:00 - 11:45 AM
Gustavo Marquez
Cryo-TEM
10:15- 11:00 AM
Fernando Mendoza
Cryo dual-beam techniques



11:00 - 11:45 AM
Francisco Capani
Serial section reconstructions: biological applications


11:50 GROUP PHOTOGRAPH
10:15 - 11:00 AM
Domingo Ferrer
Applications of Aberration-Corrected Scanning-TEM


11:00 - 11:45 PM
Wim Voorhout
Fernando Mendoza

Introduction to 3D-imaging in Life Sciences
10:15 - 11:00 AM
Francisco Capani
Correlative Fluorescent Microscopy




11:00 -11:45 AM
Fredi Sanchez
Freeze-etching/fracture


11:45 - 12:00 AM
Antonio Pinto
DBE´s PSSC Labs Computer Cluster
12:00 - 1:00 PM Lunch 12:00 - 1:00 PM Lunch 12:00 - 1:00 PM Lunch 12:00 - 1:00 PM Lunch
1:00 - 6:00 PM
3:30 - 4:00 PM Coffee break
1:00 - 6:00 PM
3:30 - 4:00 PM Coffee break
1:00 - 6:00 PM
3:30 - 4:00 PM Coffee break
1:00 - 6:00 PM
3:30 - 4:00 PM Coffee break
Rapid freezing
Wim Busing

Cryo-TEM
Wim Busing


Digital Imaging
Gustavo Marquez




Cryo-ultramicrotomy
Wim Voorhhout
Maristela Granados

Inmunolabelling

Wim Voorhhout
Maristela Granados
Jose Reinaldo Guerrero
Electron tomography
Gustavo Marquez
Wim Busing
Wim Voorhout 
Image processing
3D-reconstruction

Lorenzo Alamo
Antonio Pinto

6:15 - 7:00 PM

Wind up meeting
*Participants feedback
**Further developments in Cryo-EM/3D-Reconstruction
***Cryo-EM in Latin America
****Workshop Closure
6:00 - 7:00 PM Cocktail 6:00 - 7:00 PM Dinner 6:00 - 7:00 PM Dinner 7:00 PM Party




* Small particles to be used in the practices: TMV, liposomes or striated muscle thick filaments.


Location of the different activities
DBE
DBE
DBE
DBE
DBE
DBE
IVIC
IVIC
IVIC
Humberto Fernández-Morán Hall
CLAFCME
 
IVIC´s Open Museum
Sculpture #67

Círculo Espacial
(Spatial circle)
by
Freddy Benavides

DBE´s Garden

Karl Gaede Auditorium
EM Lab #206

FEI CM-120 cryo-EM

EM Lab #209

FEI VitRobot
RMC cryo- ultramicrotome

Computing  room
# 219

Computer Cluster
SGI workstation

Computer Center

15 dedicated Linux RH9 computers
 Restaurant
Casa 33 Residences
DBE: Departamento de Biología Estructural, IVIC (IVIC´s Department of Structural Biology)
Casa 33 - DBE distance: 1 Km      Casa 33 - Restaurant : 0.4 Km      DBE - Computer Center: 1.3 Km


Autor: Fredy Benavides                     --            Título: Círculo Radial 
Ubicación: Jardín del Departamento de Biología Estructural
Medidas: 1,50 X1,50 x 0,50 m.          --          Materiales: Aluminio
Foto: Mardonio Díaz - Departamento de Fotografía Científica - IVIC



Supplementary material

Professor PDF links

Dr. Wim Busing

printed material will be distributed at the course

Dr. Wim Voorhout

PDF #1    PDF #2    PDF #3    PDF #4   PDF#5
Ing. Fernando Mendoza PDF

M. Sc. Maristela Granados

PDF #1     PDF #2
Dr. Francisco Capani
PDF #1   PDF #2
Dr. Domingo Ferrer
Abstract  PDF #1   PDF #2
Lic. Lorenzo Alamo Click here
Dr. Gustavo Marquez PDF #1    PDF #2
Ing. Antonio Pinto PDF
http://www.lam-mpi.org/
http://cryoem.berkeley.edu/~slaton/spider/compile_pgi.shtml
M. Sc. Fredi Sanchez     PDF#1   PDF #2   PDF #3 
Dr. Jose Reinaldo Guerrero K. T. Tokuyasu J. Cell. Biol. 57: 551 - 565, 1978
Dr. Raúl Padrón PDF #1       PDF #2

IVIC´s 2D-map
http://www.yv5fih.org.ve/Mapa_IVIC.jpg
Click on map to enlarge.
Casa 33 Residences labelled as Aulas CEA - Restaurant is labelled as Comedor - Computer Center is near Administración
Casa 33 - DBE distance: 1 Km      Casa 33 - Restaurant : 0.4 Km      DBE - Computer Center: 1.3 Km


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Updated September 20, 2007