1st
CLAFCME Cryo - Electron Microscopy Workshop
ORGANIZERS
|
Dr. Raúl. Padrón Department of Structural Biology, IVIC Director of CLAFCME |
M.Sc. Fredi
Sánchez |
|
Program
September 17 - 20, 2007
Supported by:
CIASEM,
CLAFCME
- Supramolecular Structure Laboratory
- Structural
Biology Department
-
IVIC
-
CLAB (UNESCO) and FEI
Company
with the kind
help of Lic.Miriam Ramos (International Coordinator of IVIC, CLAB, tel +58 212 504 1240, fax
+58
212 504 1034, e-mail: CLAB@ivic.ve),
Lic. Carmen
Delia Flores
(Administrador, Department of Structural
Biology - IVIC - MCT) and
Electronic
Engineer Francisco Gerbes (BEINCA-FEI)
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Click photo two times to enlarge.
Foto Mardonio Diaz, Servicio de
Fotografía Científica del IVIC
| Speaker |
E-mail |
|
| Dr. Wim Busing | Wim.Busing@fei.com |
Director NanoPort, FEI Company, Eidhoven, The Netherlands |
| Dr. Wim Voorhout | Wim.Voorhout@fei.com | Manager AppLab TEM, Sr
Applications Specialist NanoBiology, Applications Laboratory, FEI Company, Eidhoven, The Netherlands |
| Ing. Fernando Mendoza | Fernando.Mendoza@fei.com | Area Manager, Indirect
Channels Group, FEI Europe B.V., Eidhoven, The Netherlands International Coordinator of CLAFCME |
| Dr. Francisco Capani |
franciscocapani@yahoo.com.ar | Facultad de Medicina, Universidad de Buenos Aires, Argentina |
| Dr. Domingo Ferrer |
dferrer@che.utexas.edu | University of Texas at Austin, Texas, U.S.A. |
| M. Sc. Maristela Granados | mgranado@ivic.ve |
Department of Structural Biology, IVIC |
| Lic. Lorenzo Alamo | alamo@ivic.ve |
Department of Structural Biology, IVIC |
| Dr. Gustavo Marquez | gmarquez@ivic.ve |
Department of Structural Biology, IVIC |
| Ing. Antonio Pinto | apinto@ivic.ve |
Department of Structural Biology, IVIC |
| M. Sc. Fredi Sánchez | fsanchez@ivic.ve |
Department of Structural Biology, IVIC |
| Dr. Jose
Reinaldo Guerrero |
guerrero@ivic.ve | Department of Structural Biology, IVIC |
| Dr. Raúl Padrón | padron@ivic.ve |
Head, Department of Structural
Biology, IVIC; Director of CLAFCME |
| Participant |
E-mail |
Country |
| Silvia
Belmonte |
sbelmont@fcm.uncu.edu.ar |
Argentina |
|
Amado Quintar |
aquintar@cmefcm.uncor.edu |
Argentina |
Claudio Fader |
cfader@fcm.uncu.edu.ar | Argentina |
Francisco Capani |
franciscocapani@hotmail.com franciscocapani@yahoo.com.ar |
Argentina |
Kildare Miranda |
kmiranda@biof.ufrj.br | Brazil |
| Jonatas Amaral |
amaraljb@gmail.com |
Brazil |
Ingrid Flores |
iflores@ivic.ve |
Colombia |
| Viviana
Falcón |
viviana.falcon@cigb.edu.cu vivianit2005@yahoo.com.mx |
Cuba |
Sandra Rodriguez |
sandra.rodriguez@cnic.edu.cu | Cuba |
| Lucero Ruiz |
lucpo@colpos.mx | Mexico |
| Armando Zepeda |
azr@servidor.unam.mx | Mexico |
Violeta Garcia |
violetaggr@yahoo.es | Peru |
| Alvaro
Oliveira |
alvarobq@fcien.edu.uy |
Uruguay |
| Domingo Ferrer | dferrer@che.utexas.edu | U.S.A. |
Jose Zambrano |
jzambran@ivic.ve | Venezuela |
Luz Acevedo |
luzmacevedo@hotmail.com | Venezuela |
| Reicy
Brito |
rbrito@ivic.ve
|
Venezuela |
| Ulf
Lundberg |
ulundber@ivic.ve |
Venezuela Suecia |
| Monday 17 | Tuesday 18 | Wednesday 19 | Thursday 20 | ||
| 8:00
- 8:45 AM Breakfast |
8:00 - 8:45 AM Breakfast | 8:00 - 8:45 AM Breakfast | 8:00 - 8:45 AM Breakfast | ||
| 9:00 -9:45 AM Wim Busing Cryo-methods |
9:00 - 9:45
AM Wim Voorhhout Cryo-preparation methods Inmunogold |
9:00
- 9:45 AM Wim Voorhout Tomography |
9:00 - 9:45 AM Raul Padron Solving biological problems with 3DEM |
||
|
9:45 - 10:15 AM Coffee break |
9:45 - 10:15 AM Coffee break |
9:45 - 10:15 AM Coffee break |
9:45 - 10:15 AM Coffee break |
||
| 10:15 - 11:00 AM Wim Busing Cryo-TEM 11:00 - 11:45 AM Gustavo Marquez Cryo-TEM |
10:15- 11:00 AM Fernando Mendoza Cryo dual-beam techniques 11:00 - 11:45 AM Francisco Capani Serial section reconstructions: biological applications
|
10:15
- 11:00 AM Domingo Ferrer Applications of Aberration-Corrected Scanning-TEM 11:00 - 11:45 PM Wim Voorhout Fernando Mendoza Introduction to 3D-imaging in Life Sciences |
Francisco Capani Correlative Fluorescent Microscopy 11:00 -11:45 AM Fredi Sanchez Freeze-etching/fracture
|
||
| 12:00 - 1:00 PM Lunch | 12:00 - 1:00 PM Lunch | 12:00 - 1:00 PM Lunch | 12:00 - 1:00 PM Lunch | ||
| 1:00 - 6:00 PM 3:30 - 4:00 PM Coffee break |
1:00 - 6:00 PM 3:30 - 4:00 PM Coffee break |
1:00 - 6:00 PM 3:30 - 4:00 PM Coffee break |
1:00 - 6:00 PM 3:30 - 4:00 PM Coffee break |
||
|
Cryo-ultramicrotomy Wim Voorhhout Maristela Granados Inmunolabelling Wim Voorhhout Maristela Granados Jose Reinaldo Guerrero |
Electron
tomography Gustavo Marquez Wim Busing Wim Voorhout |
Image processing 3D-reconstruction Lorenzo Alamo Antonio Pinto 6:15 - 7:00 PM Wind up meeting *Participants feedback **Further developments in Cryo-EM/3D-Reconstruction ***Cryo-EM in ****Workshop Closure |
||
| 6:00 - 7:00 PM Cocktail | 6:00 - 7:00 PM Dinner | 6:00 - 7:00 PM Dinner | 7:00 PM Party | ||
* Small
particles to be used in the practices: TMV, liposomes or striated
muscle thick
filaments.
| DBE |
DBE |
DBE |
DBE |
DBE |
DBE |
IVIC |
IVIC |
IVIC |
| Humberto Fernández-Morán Hall |
CLAFCME IVIC´s Open Museum Sculpture #67 Círculo Espacial (Spatial circle) by Freddy Benavides DBE´s Garden |
Karl
Gaede Auditorium |
EM Lab #206 FEI CM-120 cryo-EM |
EM Lab #209 FEI VitRobot RMC cryo- ultramicrotome |
Computing room # 219 Computer Cluster SGI workstation |
Computer Center 15 dedicated Linux RH9 computers |
Restaurant |
Casa 33 Residences |
Casa 33 - DBE distance: 1 Km Casa 33 - Restaurant : 0.4 Km DBE - Computer Center: 1.3 Km
Autor: Fredy
Benavides
-- Título: Círculo Radial
Ubicación: Jardín
del Departamento de Biología Estructural
Medidas: 1,50
X1,50 x 0,50 m.
-- Materiales: Aluminio
Foto: Mardonio
Díaz - Departamento de
Fotografía Científica - IVIC
| Professor | PDF links |
|
Dr. Wim Busing |
printed
material will be distributed at the course |
|
Dr. Wim Voorhout |
PDF #1
PDF
#2 PDF #3
PDF #4
PDF#5 |
| Ing. Fernando Mendoza | PDF |
|
M. Sc. Maristela Granados |
PDF #1
PDF
#2 |
| Dr. Francisco Capani |
PDF
#1 PDF #2 |
| Dr. Domingo Ferrer |
Abstract
PDF
#1 PDF #2 |
| Lic. Lorenzo Alamo | Click here |
| Dr. Gustavo Marquez | PDF #1 PDF #2 |
| Ing. Antonio Pinto | PDFhttp://www.lam-mpi.org/ http://cryoem.berkeley.edu/~slaton/spider/compile_pgi.shtml |
| M. Sc. Fredi Sanchez | PDF#1 PDF #2 PDF #3 |
| Dr. Jose Reinaldo Guerrero | K.
T. Tokuyasu J. Cell. Biol. 57: 551 - 565, 1978 |
| Dr. Raúl Padrón | PDF #1 |
IVIC´s 2D-map
Click on map to enlarge.
Casa 33 Residences labelled as Aulas CEA - Restaurant is labelled as Comedor - Computer Center is near Administración
Casa 33 - DBE distance: 1 Km Casa 33 - Restaurant : 0.4 Km DBE - Computer Center: 1.3 Km
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